isolation of biosurfactant producing bacteria from poultry breast skin
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abstract
background biosurfactants are surface-active compounds produced by some microorganisms. objectives in this study, we collected surface skin samples from breast of poultry (chicken, turkey, and, quail) and screened for biosurfactant-producing bacteria. we also determined the genera of cultured strains. materials and methods 33 hemolytic bacterial strains (15, 11, and 7 isolates from chicken, turkey, and quail, respectively) were isolated; oil spreading (os) and bioemulsifying activities were measured for all isolates. results two isolates of chicken (6.06%), three of turkey (9.1%), and three of quail (9.1%) were positive in all examinations (hemolysis, emulsification index (e24) and oil spreading). in total, eight isolates (24.24%) were positive in all examinations, out of them, seven isolates (87.5%) were gram positives, mainly belonged to bacillus spp., staphylococcus spp. and lactobacillus spp. 31 isolates (93.9%) (out of 33 hemolytic isolates) were positive in oil spreading test while only eight isolates (24.24%) were positive in e24 test. conclusions the results showed that biosurfactant-producing bacteria are distributed in breast skin surface of examined birds. further investigation about the composition of biosurfactants and phylogenetic determination of biosurfactant producing bacteria is suggested.
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Journal title:
jundishapur journal of natural pharmaceutical productsجلد ۷، شماره ۳، صفحات ۹۳-۹۶
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